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4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid HEPES |
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Alias: |
N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid |
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CAS NO.: |
7365-45-9 |
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Molecular formula: |
C8H18N2O4S |
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Structure: |
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Specifications: |
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Sepcification |
Standard |
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Appearance |
White Crystalline Powder |
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Assay % |
≥99.5 |
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Solubility(1m Aq) |
Clear,colorless solution |
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PH(1% aq) |
5.0-6.5 |
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Heavy Metals (PPM) |
≤10 |
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Loss On Drying % |
≤1.0 |
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UV 260nm(1m aq) |
≤0.1 |
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UV 280nm(1m aq) |
≤0.07 |
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Fe Iron ppm |
≤5 |
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Sulfate Ion % |
≤0.05 |
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Purpose: |
4- hydroxyethyl piperazine ethyl sulfonic acid is a biological buffer; The reaction of the separation and analysis of RNA component buffer, buffer, hybridization buffer in advance; Used for RNA and T4RNA ligase 3'- terminal marking; Used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kits. To uninfected cells function. It is a kind of hydrogen ion buffer, can control the scope of the pH value of the constant for a long time. Use the final concentration of 10-50 tendency for L, general culture medium containing 20 tendency LHEPES buffering capacity could be achieved. |
